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Image Search Results
Journal: International Journal of Oncology
Article Title: Butyrate supplementation regulates expression of chromosome segregation 1-like protein to reverse the genetic distortion caused by p53 mutations in colorectal cancer
doi: 10.3892/ijo.2022.5354
Figure Lengend Snippet: Different CSE1L expression levels in the colon cell lines. (A) Relative mRNA expression levels of CSE1L in CCD-18Co and FHC cells. (B) Relative mRNA expression levels of CSE1L in the CRC cell lines. (C) Relative mRNA expression levels of CSE1L in overconfluent Caco-2 cells. (D) Relative mRNA expression levels of c-Myc in overconfluent Caco-2 cells. (E) Protein expression level of CSE1L and c-Myc in overconfluent Caco-2 cells. (F) Relative mRNA expression levels of CSE1L in HCT116 cells with or without p53 expression. (G) Protein expression level of CSE1L in HCT116 cells with or without p53 expression. (H) Knockdown efficacy of p53 by mRNA level in HCT116 p53 +/+ cells. (I) Relative mRNA expression levels of CSE1L in HCT116 cells without or with p53 knockdown. (J) Protein expression levels of CSE1L in 5-FU-treated wild-type HCT116 cells. * P<0.05, ** P<0.01 and *** P<0.001. CRC, colorectal cancer; 5-FU, 5-fluouracil; shLuc, lentiviral construct targeting luciferase; shp53, lentiviral construct targeting p53; wt, wild-type; sh, short hairpin; CSE1L, chromo-some segregation 1-like protein.
Article Snippet: In addition, three human CRC cell lines (LS 174T, cat. no. CL-188; T84, cat. no. CCL-248; HCT116 p53 +/+ , cat. no. CCL-247; all from ATCC) expressing wild-type p53, two
Techniques: Expressing, Knockdown, Construct, Luciferase
Journal: International Journal of Oncology
Article Title: Butyrate supplementation regulates expression of chromosome segregation 1-like protein to reverse the genetic distortion caused by p53 mutations in colorectal cancer
doi: 10.3892/ijo.2022.5354
Figure Lengend Snippet: Effect of butyrate supplementation and CSE1L overexpression on the genetic distortion caused by p53 mutations in colorectal cancer. Butyrate supplementation downregulates expression of CSE1L in p53-mutated CRC cells to mitigate the maligancy of CRC. CSE1L, chromosome segregation 1-like protein.
Article Snippet: In addition, three human CRC cell lines (LS 174T, cat. no. CL-188; T84, cat. no. CCL-248; HCT116 p53 +/+ , cat. no. CCL-247; all from ATCC) expressing wild-type p53, two
Techniques: Over Expression, Expressing